In vitro and in silico analysis of phytochemical compounds of 96% ethanol extract of semanggi (Marsilea crenata Presl.) leaves as a bone formation agent

Aditama, Agnis P.R., Ma'arif, Burhan, Laswati, Hening and Agil, Mangestuti (2021) In vitro and in silico analysis of phytochemical compounds of 96% ethanol extract of semanggi (Marsilea crenata Presl.) leaves as a bone formation agent. Journal of Basic and Clinical Physiology and Pharmacology, 32 (4). pp. 881-887. ISSN 2191-0286

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Abstract

Objectives: Osteoporosis is the result of an imbalance in the rate of bone resorption and bone formation due to a decrease in estrogen. Phytoestrogens are plant compounds with structures and functions similar to estrogen. Phytoestrogens that bind to estrogen receptors in bone cells are able to modulate bone formation. Semanggi (Marsilea crenata Presl.) is a plant that contains phytoestrogens. The purpose of this study was to observe the expression of osteocalcin and predict the content of extract phytoestrogens through a computer simulation study to study the bone formation activity of the 96% ethanol extract of M. crenata leaves on hFOB 1.19 cells. Methods: hFOB 1.19 cells were cultured in 24-well microplates, and 96% ethanol extract of M. crenata Presl. leaves was added at 62.5, 125 and 250 ppm. The expression of osteocalcin was analyzed using CLSM immunocytochemistry. Using PyRx 0.8 software and 1EREprotein for molecular docking, the compound was analyzed by computer. Results: The 96% ethanol extract of M. crenataPresl.leaves can increase the expression of osteocalcin, the optimal dose is 125 ppm, and p<0.05 is 881.658 AU. In silico study was obtained six compounds that showed similar activity 17β-estradiol as ER-βagonists. Conclusions: The 96% ethanol extract of M. crenata Presl. leaves contain six compounds that are thought to be phytoestrogens and ER-βagonists, and play a role in increasing bone formation activity and have the potential to be used as an oral drug.

Item Type:	Journal Article
Keywords:	one formation; hFOB 1.19 cell;MarsileacrenataPresl; osteocalcin; phytoestrogens
Subjects:	11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences > 111504 Pharmaceutical Sciences
Divisions:	Faculty of Medical and Health Sciences > Department of Pharmacy
Depositing User:	Burhan Ma'arif
Date Deposited:	08 Jul 2021 13:34

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