Immobilization of trypsin onto porous methacrylate-based monolith for flow-through protein digestion and its potential application to chiral separation using liquid chromatography

Amalia, Suci ORCID: https://orcid.org/0000-0002-8656-8574, Angga, Stevin C., Iftitah, Elvina D., Septiana, Dias, Anggraeny, Baiq Octaviana D, Warsito, Warsito, Hasanah, Aliya Nur and Sabarudin, Akhmad (2021) Immobilization of trypsin onto porous methacrylate-based monolith for flow-through protein digestion and its potential application to chiral separation using liquid chromatography. Heliyon, 7 (8). pp. 1-11. ISSN 24058440

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Abstract

Monolithic columns for analytical applications have attracted the researcher’s attention. In this work, the laboratory-made organic-polymer monolithic column is modified with trypsin and further applied as a nanobiocatalyst microreactor and a stationary phase for separating chiral compounds by liquid chromatography. The monolith was synthesized by in-situ copolymerization of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EDMA) or trimethylolpropane trimethacrylate (TRIM) as a crosslinking agent, with porogen of 1,4-butanediol/propanol/water (4:7:1 v/v) and AIBN as the radical polymerization initiator inside PEEK and silicosteel tubings (1.0 mm i.d × 100 mm) at 60oC for 12 h. A total monomer ratio (%T) and crosslinking agent (%C) of 40:25 and 28:12 were applied to prepare poly-(GMA-co-EDMA) and poly-(GMA-co-TRIM), respectively. The produced monoliths were further modified by introducing trypsin (10 mg/L) through the ring-opening reaction of the epoxide group existing in the monolithic column. The trypsin-immobilized poly-(GMA-co-EDMA) monolithic column was applied as the nanobiocatalyst microreactor for online/flow-through and rapid digestion of β-casein sample into its peptide fragments. The trypsin-immobilized poly-(GMA-co-TRIM) column has potential application to be used as the HPLC stationary phase for the separation of R/S-citronellal enantiomers.

Item Type:	Journal Article
Keywords:	monolith, trypsin, HPLC, chiral stationary phase, microreactor
Subjects:	03 CHEMICAL SCIENCES > 0301 Analytical Chemistry > 030108 Separation Science 03 CHEMICAL SCIENCES > 0305 Organic Chemistry > 030503 Organic Chemical Synthesis 03 CHEMICAL SCIENCES > 0301 Analytical Chemistry 03 CHEMICAL SCIENCES > 0305 Organic Chemistry
Divisions:	Faculty of Mathematics and Sciences > Department of Chemistry
Depositing User:	Mrs Suci Amalia
Date Deposited:	16 Nov 2021 22:47

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