Aktivitas antikanker dan mekanisme farmakologi ekstrak dan fraksi benalu nangka (Macrosolen cochinchinensis) pada sel kanker payudara T47D

Indradmojo, Christyaji (2016) Aktivitas antikanker dan mekanisme farmakologi ekstrak dan fraksi benalu nangka (Macrosolen cochinchinensis) pada sel kanker payudara T47D. Research Report. Lembaga Penelitian dan Pengabdian kepada Masyarakat UIN Maulana Malik Ibrahim, Malang. (Unpublished)

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Abstract

Indonesia are country with various types of species parasites, Which one parasites that has the potential an anticancer is benalu nangka ( Macrosolen cochinchinensis ).This study aims to know the level of its cytotoxicity extract and fraction of the leaves benalu nangka to cell line breast cancer t47d and to knows mechanism pharmacology by test apoptosis uses double staining and flowcytometry methods. Separation compound active leaves of benalu nangka was done with the methods extraction maceration use a solvent ethanol 96 %, fractionated with n-heksana , chloroform , and ethyl acetate . Each extract and factions were tested in toxicity level against breast cancer t47d cell line. The extract have highest anticancer activity tested mechanism induction apoptosis by double staining and flowcytometry. The result showed that cytotoxcity of ethyl acetate fraction is higher potential than the other fraction. The value of IC50 extract ethanol , n-heksana faction , chloroform faction , ethyl acetate faction , and the water respectively are 362,8; 926; 356,8; 314,8; and 2243 ug/ml. The results of the flowcytometry showed that T47D cell line having 52,41 % of early apoptosis , 35,98 % late apoptosis and 4,90 % necrosis and had some abstacles the cycle of cells in the cycle Go-G1 and phase S , and also having apoptosis in phase M1.

Item Type: Research (Research Report)
Subjects: 11 MEDICAL AND HEALTH SCIENCES > 1112 Oncology and Carcinogenesis > 111204 Cancer Therapy (excl. Chemotherapy and Radiation Therapy)
Divisions: Research and Community Service (Lembaga Penelitian dan Pengabdian kepada Masyarakat)
Depositing User: Miftahus Sholehudin
Date Deposited: 10 Jan 2017 06:30

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