Decaffeinated coffee and green tea extract inhibit foam cell atherosclerosis by lowering inflammation and improving cholesterol influx/efflux balance through upregulation of PPARγ and miR-155 [version 1; peer review: awaiting peer review]

Rachmawati, Ermin ORCID: https://orcid.org/0000-0003-1045-7066, Rohman, Mohammad Saifur, Sargowo, Djanggan, Kalsum, Umi, Lyrawati, Diana and Lukitasari, Mifetika (2021) Decaffeinated coffee and green tea extract inhibit foam cell atherosclerosis by lowering inflammation and improving cholesterol influx/efflux balance through upregulation of PPARγ and miR-155 [version 1; peer review: awaiting peer review]. F1000Research, 10 (1175). pp. 1-13. ISSN 20461402

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Abstract

Background: Foam cell is the marker of initial phase of atherosclerosis and determine the development of advance atherosclerotic plaque which is stimulated by the presence of inflammation due to high lipid burden in macrophage. The combination of coffee and tea was suggested to have a role in foam cell inhibition.
Objective: this study investigated the role of decaffeinated coffee and green tea extract on foam cell formation through modulation of inflammation process and normalisation of lipid influx/efflux in M-CSF and ox-LDL-stimulated macrophages.
Material and method: Light roasted coffee and green tea leaves were extracted using infusa method and also underwent decaffeination process with active carbon and infusa method, respectively. The cell was administered with the decaffeinated coffee and green tea extract at different concentration (160/160 and 320/320μg/ml). Foam cell formation was examined with ORO staining and lipid absorbance after eluted with isopropanol. Observation of lipid influx/efflux mechanism was represented by semiquantitative CD36/ABCA1 expression using immunofluorescence technique. The inflammation process was observed by quantify the inflammatory/anti-inflammatory marker such as TNFα and IL10 using ELISA. The expression and activity of PPARγ was assessed with PCR and ELISA, respectively. The expression of miR-155 was examined using qPCR.
Results: Decaffeinated coffee and green tea extract significantly inhibited foam cell number (p=0,000); reduced CD36 expression (p=0,000) and TNFα secretion (p=0,000). This combination also increased PPARγ expression (p=0,00) and activity (p=0,001), miR-155 expression (p=0,000), and IL10 production (p=0,000).

Item Type:	Journal Article
Keywords:	coffee; green tea; foam cell; inflammation; miR-155; PPARγ
Subjects:	11 MEDICAL AND HEALTH SCIENCES > 1102 Cardiorespiratory Medicine and Haematology > 110201 Cardiology (incl. Cardiovascular Diseases)
Divisions:	?? JDok ??
Depositing User:	dr Ermin rachmawati
Date Deposited:	01 Dec 2021 08:54

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