Analisis sidik jari kromatografi lapis tipis tanaman Anting-anting (Acalypha indica L.)

Laksono, Muhammad Teguh and Hayati, Elok Kamilah (2021) Analisis sidik jari kromatografi lapis tipis tanaman Anting-anting (Acalypha indica L.). Alchemy, 9 (2). pp. 54-62. ISSN 2086-1710

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Abstract

Anting-anting plant (Acalypha indica L.) is a plant that the community has used as raw material for herbal medicine. Anting-anting plants contain chemical compounds, so it is difficult to ensure these plants' safety and quality control. The research objective was to validate the method by knowing the stability, specificity, precision, and rigidity of the chromatographic profile of alkaloid compounds in the TLC analysis of the anting-anting extract. Extraction was carried out using ethyl acetate solvent for 20 minutes with a frequency of 42 kHz. The eluent used is cyclohexane: toluene: diethylamine (75:15:10) with Dragendorff reagent. The method of validation was acceptable according to the criteria except for the stability test of the analyte during chromatography. The instability of the analyte is indicated that not all spots form a straight diagonal line. The specificity of the method was carried out by comparing the fingerprint patterns of the leaves of the anting-anting with ekor kucing (Acalypha hibsida Farm.) and teh-tehan plants (Acalypha siamensis). The fingerprint profile showed a yellow stain (Rf = 0.39) on the leaves of the anting-anting, which did not appear in the fingerprint profiles of the two comparison samples.

Item Type:	Journal Article
Keywords:	anting-anting; fingerprint analysis; thin layer chromatography; method validation
Subjects:	03 CHEMICAL SCIENCES > 0301 Analytical Chemistry
Divisions:	Faculty of Mathematics and Sciences > Department of Chemistry
Depositing User:	Elok Kamilah Hayati
Date Deposited:	14 Dec 2021 09:01

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