Effective combination of Nigella sativa and Trigonella foenum-graecum seed extract on wound healing in diabetic mice

Susilowati, Retno ORCID: https://orcid.org/0000-0001-6514-1603 and Rohmanningrum, Ulfa Maynisa (2023) Effective combination of Nigella sativa and Trigonella foenum-graecum seed extract on wound healing in diabetic mice. Jurnal Biodjati, 8 (1). pp. 106-116. ISSN 2541-4208

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Abstract

High levels of free radicals in diabetic wounds often cause chronic inflammation. Nigella sativa and Trigonella foenum-graecum seed extracts are rich in antioxidants and have anti-inflammatory effects. Trigonella foenum-graecum seed extracts can also accelerate the reepithelialization process in wound healing. This study aimed to determine the combination of ethanol extract of Nigella sativa and Trigonella foenum-graecum seeds on wound length and the number of neutrophils, macrophages, fibroblasts, and collagen density in the incision wound of diabetic mice induced by streptozotocin. T. This study used three types of controls, namely Normal (Normal mice, HPMC 3%), DM (Diabetic mice, HPMC 3%), and Iodine (Diabetic mice, Povidone Iodine 10%). Topical treatment with 70% ethanol extract ointment combined with Nigella sativa and Trigonella foenum-graecum in various variations, namely N (20%:0%), T (0%:10%), NT (10%:5%), Observations of the wound healing process were carried out on 3rd, 7th and 14th day. Data were analyzed using the One Way ANOVA and Duncan Multiple Range Test. The study's results by observing the morphology of the wound length on day 7th day showed that the combination treatment of extracts of Nigella sativa and Trigonella foenum-graecum resulted in the shortest wound, which was significantly different from the wound in untreated diabetic mice. In addition, diabetic wounds without treatment showed infection, and on the contrary, the infection did not occur in wounds treated with a combination of extracts of Nigella sativa and Trigonella foenum-graecum. The results of wound histology observations showed that the combination treatment of extracts of Nigella sativa and Trigonella foenum-graecum showed a decrease in inflammation which was indicated by a significant decrease in the number of neutrophil cells, macrophages, and accelerated reepithelialization of wound healing as indicated by a significant increase in the number of fibroblasts and collagen density since the third day of treatment.

Item Type:	Journal Article
Keywords:	Diabetes Mellitus (DM); Nigella sativa; Trigonella foenum-graecum; wound healing
Subjects:	06 BIOLOGICAL SCIENCES > 0606 Physiology > 060603 Animal Physiology - Systems 11 MEDICAL AND HEALTH SCIENCES > 1104 Complementary and Alternative Medicine > 110499 Complementary and Alternative Medicine not elsewhere classified 11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences > 111501 Basic Pharmacology
Divisions:	Faculty of Mathematics and Sciences > Department of Biology
Depositing User:	Retno Susilowati
Date Deposited:	14 Jun 2023 20:54

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