Karakterisasi dan uji aktivitas antibakteri cell free supernatant isolat bakteri asam laktat pada produk susu fermentasi di Malang

Firdausy, Alif Firman ORCID: https://orcid.org/0000-0001-7373-0336, Walidah, Ziyana, Mufidah, Kurnia, Ningrum, Nofita Diyah, Rahmadhany, Azizah Nurul and Adila, Afnan (2021) Karakterisasi dan uji aktivitas antibakteri cell free supernatant isolat bakteri asam laktat pada produk susu fermentasi di Malang. Research Report. UIN Maulana Malik Ibrahim Malang, Malang. (Unpublished)

Preview

Text 15089.pdf Download (1MB) | Preview

Abstract

Yogurt and kefir are well-known fermented dairy products that provide many benefits to human health. Microorganisms contained in yogurt and kefir play an important role to produce bioactive metabolites, including eradicating pathogens. This study presents the antimicrobial activity of 12 strains isolated from kefir and yogurt samples obtained in Malang, Indonesia. Strain K3 from kefir shows the highest antimicrobial activity with the diameter of the inhibitory zone was 8.11±0.07 mm against Escherichia coli and 8.20±0.17 mm against Staphylococcus aureus. Phenotypic characterization through Gram-staining, endospore-forming, motility, catalase, oxidase, and carbohydrate fermentation test, and genotypic identification through the 16S rRNA gene sequencing and phylogenetic analysis was performed to identify the species of isolate. Based on the result, K3 was characterized as non-motile Gram-negative bacilli bacteria, tested negative in endospore formation, catalase, and oxidase. According to the nucleotide sequence of 16S rRNA gene, the isolate was classified as Staphylococcus saprophyticus strain UTI-045 with 100% of similarity. Furthermore, the GC-MS analysis of the strain’s cell-free supernatant (CFS) reveals 3,6-Dioxa-2, 7-disilaoctane, 2,2,4,7,7-pentamethyl- (Rt=9.449 min); and Propanoic acid, 2-[(trimethylsilyl)oxy]-, trimethylsilyl ester (Rt=11.328 min) as the active metabolites.

Item Type:	Research (Research Report)
Keywords:	fermentation; dairy product; coagulase-negative staphylococci; antimicrobial
Subjects:	11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110801 Medical Bacteriology 11 MEDICAL AND HEALTH SCIENCES > 1111 Nutrition and Dietetics > 111102 Dietetics and Nutrigenomics
Divisions:	Faculty of Medical and Health Sciences > Department of Pharmacy
Depositing User:	Alif Firman Firdausy
Date Deposited:	17 Jun 2023 04:57

Downloads

Origin of downloads

Actions (login required)

View Item