Phytochemical, metabolite compound, and antioxidant activity of Clinacanthus nutans leaf extract from Indonesia

Susanti, Nurlaili, Mustika, Arifa, Khotib, Junaidi, Mutiah, Roihatul ORCID: https://orcid.org/0000-0002-8196-9029 and Rochmanti, Maftuchah (2023) Phytochemical, metabolite compound, and antioxidant activity of Clinacanthus nutans leaf extract from Indonesia. Science and Technology Indonesia, 8 (1). pp. 38-44. ISSN 2580-4391

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Abstract

The antioxidant properties from medicinal plants have protective effects against oxidative stress-linked diseases. Their antioxidant capacities are related to phenolic compounds, including phenols and flavonoids. Clinacanthus nutans, a native to Asia, including Malaysia, Thailand, and Indonesia, are used for treating diabetes and other diseases, but the exploration of the plant in Indonesia is still limited. Therefore, this study aimed to reveal the phytochemical constituents, metabolic compounds, and antioxidant activity of C. nutans extracts. Dried leaf powder was extracted with 70% ethanol and aqueous, each extract was subjected to qualitative phytochemical screening. Subsequently, total phenolic and flavonoid content was calculated using Folin-Ciocalteu and AlCl3 colorimetric assay. The antioxidant activity was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. The profiles of phenolic and flavonoid compounds of 70% ethanolic extract were investigated by LC-MS/MS. Phytochemical screening showed that the extracts contain phenolic, flavonoid, tannin, and saponin. The total phenolic content of the 70% ethanol and aqueous extract was 4.14±1.9% mgGAE/g and 3.89±3.2% mgGAE/g, respectively. Meanwhile, the total flavonoid content obtained was 0.19±1.0% mgQE/g and 0.03±0.5% mgQE/g for 70% ethanol and aqueous extracts, respectively. Both extracts showed high antioxidant activity, with DPPH IC50 30.57

Item Type:	Journal Article
Keywords:	Clinacanthus nutans; DPPH; LC-MS/MS; total flavonoid content; total phenolic content
Subjects:	03 CHEMICAL SCIENCES > 0301 Analytical Chemistry > 030199 Analytical Chemistry not elsewhere classified 11 MEDICAL AND HEALTH SCIENCES > 1104 Complementary and Alternative Medicine > 110499 Complementary and Alternative Medicine not elsewhere classified 03 CHEMICAL SCIENCES > 0301 Analytical Chemistry
Divisions:	Faculty of Medical and Health Sciences > Department of Medical Education
Depositing User:	Dr. M.Kes. Roihatul Muti'ah
Date Deposited:	20 Sep 2023 14:30

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