The analysis of coffee-green tea-turmeric combination against cardiac-metabolic syndrome using metabolite profiling, gene expression, and in silico approach

Rachmawati, Ermin ORCID: https://orcid.org/0000-0003-1045-7066, Rohman, M. Saifur, Widodo, Nashi, Lukitasari, Mifetika, Nugroho, Dwi A., Hermanto, Feri and Kholis, Mukhamad N. (2023) The analysis of coffee-green tea-turmeric combination against cardiac-metabolic syndrome using metabolite profiling, gene expression, and in silico approach. Journal of Pharmacy and Pharmacognosy Research, 11 (6). pp. 961-974. ISSN 0719-4250

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Abstract

Context: The development of functional drinks to inhibit oxidative stress and inflammation as a critical process in inducing heart damage in metabolic
syndrome is required. Coffee, tea, and turmeric have all been shown to offer health advantages.
Aims: To investigate the effect of coffee, green tea, turmeric extract (ECGTT) against cardiac-metabolic syndrome (MetS).
Methods: The secondary metabolites from coffee, green tea, and turmeric were identified using LC-HRMS. Male Sprague–Dawley rats were divided into four
groups (n = 4) representing normal, MetS, MetS with ECGTT treatment doses: 300/100/150 mg/BW and 300/100/250 mg/BW group. Upon the end of treatment
periods, expression of tumor necrosis factor-alpha (TNFα), interleukin-6 (IL-6), nuclear factor kappa B (NF-κB), NADPH oxidase (NOX2), SERCA2a were
measured from the heart. A computational approach including network pharmacology, protein-protein interaction (PPI) network, molecular docking, and
dynamic was performed to understand the molecular mechanism of ECGTT against cardiac damage in MetS.
Results: Chlorogenic acid (CGA), epigallocatechin gallate (EGCG), and curcumin were identified as the main metabolites in ECGTT. The ECGTT administration
decreased the TNFα, IL-6, NF-κB, and NOX2 and increased SERCA2a expression(p<0.05). Moreover, the PPI result suggested that angiotensin II receptor type 1
(AGTR1) was the key regulator of cardiac injury-MetS induced. CGA, EGCG, and curcumin bind to AGTR1 with smaller binding energy than metformin and
showed stability of structure and interaction among those metabolites into AGTR1.
Conclusions: Coffee, green tea, and turmeric might prevent heart dysfunction in MetS through modulation of oxidative stress and inflammation.

Item Type:	Journal Article
Keywords:	calcium handling; coffee; green tea; inflammation; oxidative stress; turmeric
Subjects:	11 MEDICAL AND HEALTH SCIENCES > 1102 Cardiorespiratory Medicine and Haematology > 110201 Cardiology (incl. Cardiovascular Diseases) 11 MEDICAL AND HEALTH SCIENCES > 1104 Complementary and Alternative Medicine > 110499 Complementary and Alternative Medicine not elsewhere classified
Divisions:	Faculty of Medical and Health Sciences > Department of Medical Education
Depositing User:	dr Ermin rachmawati
Date Deposited:	08 Nov 2023 07:54

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