10-Gingerol as an inducer of apoptosis through HTR1A in cumulus cells: In-vitro and in-silico studies

Kiptiyah, Kiptiyah and Widodo, Widodo and Ciptadi, Gatot and Aulanni'am, Aulanni'am and Widodo, Mohammad Aris and Sumitro, Sutiman Bambang (2017) 10-Gingerol as an inducer of apoptosis through HTR1A in cumulus cells: In-vitro and in-silico studies. Journal of Taibah University Medical Sciences, 12 (5). pp. 397-406. ISSN 16583612

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Abstract

Objectives Cumulus cells play a crucial role as essential mediators in the maturation of ova. Ginger contains 10-gingerol, which induces apoptosis in colon cancer cells. Based on this hypothesis, this study aimed to determine whether 10-gingerol is able to induce apoptosis in normal cells, namely, cumulus cells. Methods This study used an in vitro analysis by culturing Cumulus cells in M199 containing 10-gingerol in various concentrations (12, 16, and 20 μM) and later detected early apoptotic activity using an Annexin V-FITC detection kit. Result The in vitro data revealed that the number of apoptosis cells increased along with the period of incubation as follows: 12 μM (63.71% ± 2.192%); 16 μM (74.51% ± 4.596%); and 20 μM (78.795% ± 1.435%). The substance 10-gingerol induces apoptosis in cumulus cells by inhibiting HTR1A functions and inactivating GSK3B and AKT-1. Conclusions These findings indicate that further examination is warranted for 10-gingerol as a contraception agent.

Item Type: Journal Article
Keywords: 10-Gingerol; Apoptosis; Cumulus cells; HTR1A; In silico; In vitro; (10) gingerol; aphios; fluorescein isothiocyanate; glucose; glycogen synthase kinase 3beta; lipocortin 5; protein kinase B; serotonin; serotonin receptor, antineoplastic activity; apoptosis; Article; cell culture; computer model; concentration response; cumulus cell; drug effect; female; human; human cell; in vitro study; incubation time; protein function
Divisions: Faculty of Mathematics and Sciences > Department of Biology
Depositing User: Heni Kurnia Ningsih
Date Deposited: 28 May 2018 03:45

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